Reporter

Part:BBa_K1975008:Experience

Designed by: Joachim Steen Larsen   Group: iGEM16_UNIK_Copenhagen   (2016-10-13)


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Applications of BBa_K1975008

We have validated this BioBrick to be a ampicillin resistent marker gene. We used it in E. coli at different concentrations, and found that it worked at a concentrations of 100µg/mL, and it worked very well (see main page). Also, the cells did not show any sign of decreased growth, meaning that this BioBrick can restore total growth in ampicillin containing medium (Fig. 1).

<img src="T--UNIK_Copenhagen--AmpR2.jpg" alt="f2" height=300px>

Figure 1: no colonies are formed on the plate with ampicillin if not they are transformed with the vector containing the AmpE gene. This means that the cells need this gene to survive on ampicillin.

User Reviews

UNIQ8c3dd8d827bb3a94-partinfo-00000000-QINU UNIQ8c3dd8d827bb3a94-partinfo-00000001-QINU